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(Plasmid #71462)


Item Catalog # Description Quantity Price (USD)
Plasmid 71462 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size (bp) 2958
  • Modifications to backbone
    Addition of hybrid human U6/bacteriophage T7 promoter, gRNA cloning site and gRNA sequence
  • Vector type
    Mammalian Expression, Bacterial Expression, CRISPR
  • Promoter human U6/phage T7

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6T7 was a gift from Willie Mark (Addgene plasmid # 71462 ; ; RRID:Addgene_71462)
  • For your References section:

    A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs. Romanienko PJ, Giacalone J, Ingenito J, Wang Y, Isaka M, Johnson T, You Y, Mark WH. PLoS One. 2016 Feb 5;11(2):e0148362. doi: 10.1371/journal.pone.0148362. eCollection 2016. PONE-D-15-48175 [pii] PubMed 26849369