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pEGFP-C2_SEPT9 i4
(Plasmid #71617)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71617 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Septin 9
  • Species
    H. sapiens (human)
  • Entrez Gene
    SEPTIN9 (a.k.a. AF17q25, MSF, MSF1, NAPB, PNUTL4, SEPT9, SINT1, SeptD1)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C2_SEPT9 i4 was a gift from Cristina Montagna & Elias Spiliotis (Addgene plasmid # 71617 ; http://n2t.net/addgene:71617 ; RRID:Addgene_71617)
  • For your References section:

    Septin 9 isoform expression, localization and epigenetic changes during human and mouse breast cancer progression. Connolly D, Yang Z, Castaldi M, Simmons N, Oktay MH, Coniglio S, Fazzari MJ, Verdier-Pinard P, Montagna C. Breast Cancer Res. 2011 Aug 10;13(4):R76. doi: 10.1186/bcr2924. 10.1186/bcr2924 PubMed 21831286