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pmCherry-C1_SEPT9 i1
(Plasmid #71622)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71622 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCherry-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Septin 9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • Entrez Gene
    SEPTIN9 (a.k.a. AF17q25, MSF, MSF1, NAPB, PNUTL4, SEPT9, SINT1, SeptD1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer mCherry-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry-C1_SEPT9 i1 was a gift from Elias Spiliotis (Addgene plasmid # 71622 ; http://n2t.net/addgene:71622 ; RRID:Addgene_71622)
  • For your References section:

    Septins promote stress fiber-mediated maturation of focal adhesions and renal epithelial motility. Dolat L, Hunyara JL, Bowen JR, Karasmanis EP, Elgawly M, Galkin VE, Spiliotis ET. J Cell Biol. 2014 Oct 27;207(2):225-35. doi: 10.1083/jcb.201405050. 10.1083/jcb.201405050 PubMed 25349260