pBbE1k-3
(Plasmid #71722)

• Purpose: tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human
• Depositing Lab: Taek Soon Lee
• Publication: Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29.

Backbone

• Vector backbone: pBbE1k
• Backbone manufacturer: JBEI
• Total vector size (bp): 6217

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: dihydropterine reductase
• Alt name: DHPR
• Species: H. sapiens (human)
• Insert Size (bp): 734
• Entrez Gene: QDPR (a.k.a. DHPR, HDHPR, PKU2, SDR33C1)
• Promoter: pTrc

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: pterin-4a-carbinolamine dehydratase
• Alt name: PCD
• Species: H. sapiens (human)
• Insert Size (bp): 314
• Entrez Gene: PCBD1 (a.k.a. DCOH, PCBD, PCD, PHS)
• Promoter: pTrc

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pY011 plasmid map
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a patent for this.
Lee, T.S., Satoh, Y., Keasling, J.D., Host cells and methods for oxidizing aromatic amino acids, PCT/US2012/031025