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pTA7001-DEST
(Plasmid #71745)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71745 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBI101
  • Backbone manufacturer
    Clontech
  • Backbone size (bp) 9000
  • Modifications to backbone
    T-DNA region of pBI101 was replaced with a new T-DNA sequence that enables dexamethasone-inducible gene expression of a gene of interest. Construction of pTA7001 is described in Aoyama, T. and Chua, N.-H. (1997) A glucocorticoid-mediated transcriptional induction system in transgenic plants. Plant J. 11, 605–612. pTA7001 was further modified to contain a GATEWAY destination cassette inserted between Xho1 and Spe1 sites.
  • Vector type
    Plant Expression
  • Promoter GAL4
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jean Greenberg at the University of Chicago.
  • Terms and Licenses

Depositor Comments

Aoyama, T. and Chua, N.-H. (1997) A glucocorticoid-mediated
transcriptional induction system in transgenic plants. Plant J.
11, 605–612

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTA7001-DEST was a gift from Roger Innes (Addgene plasmid # 71745 ; http://n2t.net/addgene:71745 ; RRID:Addgene_71745)
  • For your References section:

    The KEEP ON GOING protein of Arabidopsis recruits the ENHANCED DISEASE RESISTANCE1 protein to trans-Golgi network/early endosome vesicles. Gu Y, Innes RW. Plant Physiol. 2011 Apr;155(4):1827-38. doi: 10.1104/pp.110.171785. Epub 2011 Feb 22. 10.1104/pp.110.171785 PubMed 21343429