Purpose(Empty Backbone) Cloning vector for expression of sgRNA without PBS. It contains extra sequences for BsaI digestion for insertion of PBS
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71898||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepX330; pUC ori vector
Vector typeMammalian Expression, CRISPR
- Promoter U6
Growth in Bacteria
- Cloning method Unknown
- 5′ sequencing primer gagggcctatttcccatgattcc
- 3′ sequencing primer gccatttgtctgcagaattggc (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAC1394-pX-sgRNA-0xPBS was a gift from Albert Cheng (Addgene plasmid # 71898 ; http://n2t.net/addgene:71898 ; RRID:Addgene_71898)
For your References section:Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling. Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, Taghbalout A, Wang H. Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 Jan 15. 10.1038/cr.2016.3 PubMed 26768771