Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling.
Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, Taghbalout A, Wang H
Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 Jan 15. PubMed Article
Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, Taghbalout A, Wang H
Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 Jan 15. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71879 | pAC164-pmax-dCas9Master-VP64 | dCas9-3xGGGGS-VP64 in pmax expression vector |
| 71881 | pAC1355-pmax-NLSPUFa_VP64 | NLSPUFa_VP64 in transient expression vector pmax |
| 71882 | pAC1356-pmax-NLSPUFb_VP64 | NLSPUFb_VP64 in expression vector pmax |
| 71883 | pAC1357-pmax-NLSPUFw_VP64 | NLSPUFw_VP64 in expression vector pmax |
| 71884 | pAC1358-pmax-NLSPUFc_VP64 | NLSPUFc_VP64 in expression vector pmax |
| 71887 | pAC1364-pmax-dCas9Master_mCBPHAT | dCas9Master_mCBPHAT in pmax expression vector |
| 71888 | pAC1371-pX-sgRNA-5xPBSa | Cloning vector for expression of sgRNA-5xPBSa |
| 71889 | pAC1372-pX-sgRNA-15xPBSa | Cloning vector for expression of sgRNA-15xPBSa |
| 71890 | pAC1373-pX-sgRNA-25xPBSa | Cloning vector for expression of sgRNA-25xPBSa |
| 71891 | pAC1374-pX-sgRNA-5xPBSb | Cloning vector for transient expression of sgRNA-5xPBSb |
| 71892 | pAC1375-pX-sgRNA-15xPBSb | Cloning vector for expression of sgRNA-15xPBSb |
| 71893 | pAC1376-pX-sgRNA-25xPBSb | Cloning vector for expression of sgRNA-25xPBSb |
| 71894 | pAC1379-pX-sgRNA-5xPBSw | Cloning vector for expression of sgRNA-5xPBSw |
| 71895 | pAC1380-pX-sgRNA-5xPBSc | Cloning vector for expression of sgRNA-5xPBSc |
| 71897 | pAC1393-pmax-NLSPUFa_p65HSF1 | NLSPUFa_p65HSF1 in pmax expression vector |
| 71898 | pAC1394-pX-sgRNA-0xPBS | Cloning vector for expression of sgRNA without PBS. It contains extra sequences for BsaI digestion for insertion of PBS |
| 71899 | pAC1399-pX-sgRNA-20xPBSc | Cloning vector for expression of sgRNA-20xPBSc |
| 71900 | pAC1402-pCR8-Clover_NLSPUFa | Clover_NLSPUFa in pCR8 gateway donor vector |
| 71901 | pAC1403-pCR8-Clover_NLSPUFc | Clover_NLSPUFc in pCR8 gateway donor vector |
| 71902 | pAC1404-pCR8-mRuby2_NLSPUFa | mRuby2_NLSPUFa in pCR8 gateway donor vector |
| 71903 | pAC1405-pCR8-4xNLS_PUFa_2xNLS | NLSPUFa pCR8 gateway donor vector for insertion of N-terminal domain (SgrAI or AgeI with ClaI) and C-terminal domain (FseI PacI). Grow in dcm- cells (e.g., NEB C3040) to prepare vector for ClaI digest |
| 71904 | pAC1406-pCR8-4xNLS_PUFb_2xNLS | NLSPUFb pCR8 gateway donor vector for insertion of N-terminal domain (SgrAI or AgeI with ClaI) and C-terminal domain (FseI PacI). Grow in dcm- cells (e.g., NEB C3040) to prepare vector for ClaI digest |
| 71905 | pAC1407-pCR8-4xNLS_PUFw_2xNLS | NLSPUFw pCR8 gateway donor vector for insertion of N-terminal domain (SgrAI or AgeI with ClaI) and C-terminal domain (FseI PacI). Grow in dcm- cells (e.g., NEB C3040) to prepare vector for ClaI digest |
| 71906 | pAC1408-pCR8-4xNLS_PUFc_2xNLS | NLSPUFc pCR8 gateway donor vector for insertion of N-terminal domain (SgrAI or AgeI with ClaI) and C-terminal domain (FseI PacI). Grow in dcm- cells (e.g., NEB C3040) to prepare vector for ClaI digest |
| 71907 | pAC1410-pmax-dCas9Master_p65HSF1 | dCas9Master_p65HSF1 in pmax expression vector |
| 71908 | pAC1411-pmax-NLSPUFc_p65HSF1 | NLSPUFc_p65HSF1 in pmax expression vector |
| 71912 | pAC1415-pCR8-4xNLS_PUFa_2xNLS_mCBPHAT | 4xNLS_PUFa_2xNLS_mCBPHAT in pCR8 Gateway donor vector |
| 71913 | pAC1416-pCR8-mCBPHAT_4xNLS_PUFa_2xNLS | mCBPHAT_4xNLS_PUFa_2xNLS in pCR8 Gateway donor vector |
| 71914 | pAC1417-pmax-4xNLS_PUFa_2xNLS_mCBPHAT | 4xNLS_PUFa_2xNLS_mCBPHAT in pmax expression vector |
| 71915 | pAC1418-pmax-mCBPHAT_4xNLS_PUFa_2xNLS | mCBPHAT_4xNLS_PUFa_2xNLS in pmax expression vector |
| 71917 | pAC1420-pX-sgRNA-1xPBSa | Cloning vector for expression of sgRNA-1xPBSa |
| 71918 | pAC1421-pX-sgRNA-2xPBSa | Cloning vector for expression of sgRNA-2xPBSa |
| 71919 | pAC1422-pX-sgRNA-1xPBSb | Cloning vector for expression of sgRNA-1xPBSb |
| 71920 | pAC1423-pX-sgRNA-2xPBSb | Cloning vector for expression of sgRNA-2xPBSb |
| 71921 | pAC1424-pX-sgRNA-10xPBSb | Cloning vector for expression of sgRNA-10xPBSb |
| 71925 | pAC1425-pX-sgRNA-20xPBSb | Cloning vector for expression of sgRNA-20xPBSb |
| 71926 | pAC1426-pX-sgRNA-47xPBSb | Cloning vector for expression of sgRNA-47xPBSb |
| 71927 | pAC1427-pX-sgRNA-10xPBSw | Cloning vector for expression of sgRNA-10xPBSw |
| 71928 | pAC1428-pX-sgRNA-15xPBSw | Cloning vector for expression of sgRNA-15xPBSw |
| 71929 | pAC1429-pX-sgRNA-10xPBSc | Cloning vector for expression of sgRNA-10xPBSc |
| 71930 | pAC1430-pX-sgRNA-15xPBSc | Cloning vector for expression of sgRNA-15xPBSc |
| 73169 | pAC1445-pmax-dCas9 | dCas9 expressed in pmax vector |
| 73688 | pAC1446_pmax-Clover_PUFa | Clover_PUFa in pmax-DEST expression vector |
| 73689 | pAC1447_pmax-Clover_PUFc | Clover_PUFc in pmax-DEST expression vector |
| 73690 | pAC1448_pmax-mRuby2_PUFa | mRuby2_PUFa in pmax-DEST expression vector |
| 73691 | pAC1449_pmax-KRAB_PUFa | KRAB_PUFa in pmax-DEST expression vector |
| 73692 | pAC1450_pCR8-BFPKRAB_PUFa | BFPRKAB_PUFa in pCR8 gateway donor vector |
| 73693 | pAC1451_pmax-BFPKRAB_PUFa | BFPKRAB_PUFa in pmax-DEST expression vector |
| 73694 | pAC1452_pmax-dCas9_mCherry | dCas9_mCherry in pmax-DEST expression vector |
| 73695 | pAC1453_pX-sgTelomere-25xPBSa | Vector expressing sgTelomere-25xPBSa |
| 73696 | pAC1454_pX-sgCentromere-25xPBSc | Vector expression sgCentromere-20xPBSc |
| 73697 | pAC1392_pmax-PUFb_p65HSF1 | PUFb_p65HSF1 in pmax-DEST expression vector |