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pGLS3-5xHRE-p53(K120R/K164R)
(Plasmid #72556)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72556 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Modifications to backbone
    p53 was cloned so that it replaced luciferase. Neo cassette was inserted into BamHI site.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p53
  • Species
    H. sapiens (human)
  • Mutation
    K120R/K164R
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter E1B minimal promoter
  • Tag / Fusion Protein
    • 5XHRE (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer E1B F: 5’-agatctggtaggcgtgtacgg-3’
  • 3′ sequencing primer pGLS3 R: 5’-gtatcttatcatgtctgctcg-3’
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGLS3-5xHRE-p53(K120R/K164R) was a gift from Ester Hammond (Addgene plasmid # 72556 ; http://n2t.net/addgene:72556 ; RRID:Addgene_72556)
  • For your References section:

    Hypoxia-induced p53 modulates both apoptosis and radiosensitivity via AKT. Leszczynska KB, Foskolou IP, Abraham AG, Anbalagan S, Tellier C, Haider S, Span PN, O'Neill EE, Buffa FM, Hammond EM. J Clin Invest. 2015 Jun;125(6):2385-98. doi: 10.1172/JCI80402. Epub 2015 May 11. 10.1172/JCI80402 PubMed 25961455