Addgene - pCAG-HIF1 alpha Plasmid Data

Plasmid 21101: pCAG-HIF1 alpha

Gene/insert name:
HYPOXIA INDUCIBLE FACTOR 1ALPHA
Alt name:
HIF1A
Insert size:
2400
Species:
H. sapiens (human)
Entrez Gene:
HIF1A (HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
Vector backbone:
pCAGEN
(Search Vector Database)
Backbone manufacturer:
Addgene Plasmid 11160
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
NOT1
Site destroyed during cloning:
No
Cloning site 3':
NOT1
Site destroyed during cloning:
No
5' sequencing primer:
pCAG-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
K.W. Kim, Seoul National University
Sequence: View sequences (1)
Principal Investigator:
Connie Cepko
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: HDAC4 regulates neuronal survival in normal and diseased retinas. Chen et al (Science. 2009 Jan 9. 323(5911):256-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21101" in your Materials and Methods section.