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pCAG-HIF1 alpha
(Plasmid #21101)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCAGEN
  • Backbone manufacturer
    Addgene Plasmid 11160
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    HYPOXIA INDUCIBLE FACTOR 1ALPHA
  • Alt name
    HIF1A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Entrez Gene
    HIF1A (a.k.a. HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NOT1 (not destroyed)
  • 3′ cloning site NOT1 (not destroyed)
  • 5′ sequencing primer pCAG-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    K.W. Kim, Seoul National University
  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-HIF1 alpha was a gift from Connie Cepko (Addgene plasmid # 21101)
  • For your References section:

    HDAC4 regulates neuronal survival in normal and diseased retinas. Chen B, Cepko CL. Science. 2009 Jan 9. 323(5911):256-9. 10.1126/science.1166226 PubMed 19131628