PurposeMammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72898||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepWPRE/C1 (modified pEGFP-C1)
Backbone manufacturerModified from Clontech
- Backbone size w/o insert (bp) 4581
- Total vector size (bp) 9118
Modifications to backboneThe woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) was inserted into the BamHI site of pEGFP-C1, and EGFP was removed.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. melanogaster (fly); Avena sativa (oat)
Insert Size (bp)4537
- Promoter CMV
/ Fusion Protein
- IRES-mCherry (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
dmBACCS2VL is a slow recovery kinetic mutant of dmBACCS2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:dmBACCS2VL-IRES-dOrai-IRES-mCherry was a gift from Takao Nakata (Addgene plasmid # 72898)
For your References section:Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata T. Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021. 10.1038/ncomms9021 PubMed 26282514