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Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS.
Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata T
Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
67625hBACCS2Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch
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67626ORAI1-hBACCS2Mammalian expression of ORAI1-hBACCS2, a fusion protein of ORAI1 and human blue light-activated Ca2+ channel switch
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67627dmBACCS2-IRES-dOraiMammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai
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67628dmBACCS2NS-IRES-dOraiMammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai
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67629dmBACCS2VL-IRES-dOraiMammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai
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67630mRFP-dmBACCS2Drosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch
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72891hBACCS2-IRES-GFPMammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and GFP
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72892hBACCS2-IRES-mCherryMammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherry
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72893dmBACCS2-IRES-dOrai-IRES-GFPMammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP
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72894dmBACCS2-IRES-dOrai-IRES-mCherryMammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry
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72895dmBACCS2NS-IRES-dOrai-IRES-GFPMammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP
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72896dmBACCS2NS-IRES-dOrai-IRES-mCherryMammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry
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72897dmBACCS2VL-IRES-dOrai-IRES-GFPMammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP
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72898dmBACCS2VL-IRES-dOrai-IRES-mCherryMammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry
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