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pcDNA 3.1(Hygro) myc-hPolQ-Flag
(Plasmid #73132)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73132 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5607
  • Total vector size (bp) 13416
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Polymerase theta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7797
  • Entrez Gene
    POLQ (a.k.a. PRO0327)
  • Promoter cmv promoter
  • Tags / Fusion Proteins
    • Flag (C terminal on insert)
    • myc (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13390-5. doi: 10.1073/pnas.0910759107. Epub 2010 Jul 12. DNA polymerase theta up-regulation is associated with poor survival in breast cancer, perturbs DNA replication, and promotes genetic instability. Lemée F1, Bergoglio V, Fernandez-Vidal A, Machado-Silva A, Pillaire MJ, Bieth A, Gentil C, Baker L, Martin AL, Leduc C, Lam E, Magdeleine E, Filleron T, Oumouhou N, Kaina B, Seki M, Grimal F, Lacroix-Triki M, Thompson A, Roché H, Bourdon JC, Wood RD, Hoffmann JS, Cazaux C.
  • Terms and Licenses

Depositor Comments

We added a myc tag to the N-terminus of the gene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA 3.1(Hygro) myc-hPolQ-Flag was a gift from Agnel Sfeir (Addgene plasmid # 73132 ; http://n2t.net/addgene:73132 ; RRID:Addgene_73132)
  • For your References section:

    Mammalian polymerase theta promotes alternative NHEJ and suppresses recombination. Mateos-Gomez PA, Gong F, Nair N, Miller KM, Lazzerini-Denchi E, Sfeir A. Nature. 2015 Feb 12;518(7538):254-7. doi: 10.1038/nature14157. Epub 2015 Feb 2. 10.1038/nature14157 PubMed 25642960