PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||73425||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerKoffas Lab, Addgene Plasmid #49795
- Backbone size w/o insert (bp) 5147
Vector typeBacterial Expression, CRISPR, Synthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePromoter 3A2 (orthogonal T7-lac variant)
- Promoter P3A2 (orthogonal T7-lac variant)
- Cloning method Unknown
- 5′ sequencing primer pBRrevBam
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Constructed using degenerate site directed mutagenesis of T7-lac promoter region. Also encodes mCherry reporter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pETM6-3A2-mCherry was a gift from Mattheos Koffas (Addgene plasmid # 73425 ; http://n2t.net/addgene:73425 ; RRID:Addgene_73425)
For your References section:Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, Linhardt RJ, Koffas MA. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. 10.1093/nar/gkw231 PubMed 27079979