Purposepromoterless Tol2 vector with attR1/attR2 cassette and late SV40 polyadenylation signal
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||73484||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
Gene/Insert nameattR1/attR2 cassette
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (unknown if destroyed)
- 3′ cloning site PspOMI (not destroyed)
- 5′ sequencing primer M13-FP
- 3′ sequencing primer M13R-pUC (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:251 pTol2Dest was a gift from Nathan Lawson (Addgene plasmid # 73484)
For your References section:Gateway compatible vectors for analysis of gene function in the zebrafish. Villefranc JA, Amigo J, Lawson ND. Dev Dyn. 2007 Nov . 236(11):3077-87. 10.1002/dvdy.21354 PubMed 17948311
Generated by Addgene from full sequence supplied by depositor.