-
PurposeTo express the inhibitory scaffold gephyrin tagged to teal fluorescent protein. Expression is Cre dependant. Plasmid can also be used to make lentivirus.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 73918 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneFU-dioYFP-W
- Total vector size (bp) 12467
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameTeal-gephyrin
-
SpeciesR. norvegicus (rat)
-
Insert Size (bp)2973
-
GenBank ID268566
-
Tag
/ Fusion Protein
- Teal (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsiWI (unknown if destroyed)
- 3′ cloning site NheI (unknown if destroyed)
- 5′ sequencing primer None
- 3′ sequencing primer None (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byJerry Chen
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid can be used with the low-efficiency Cre in Plasmid 101242: pSin wPGK-Cre ( https://www.addgene.org/101242/ ).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
FU- dio Teal-gephyrin - W was a gift from Elly Nedivi (Addgene plasmid # 73918 ; http://n2t.net/addgene:73918 ; RRID:Addgene_73918) -
For your References section:
Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo. Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y, So PT, Nedivi E. Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 10.1016/j.neuron.2016.01.010. 10.1016/j.neuron.2016.01.010 PubMed 26853302