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Addgene

FU- dio Teal-gephyrin - W
(Plasmid #73918)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73918 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    FU-dioYFP-W
  • Total vector size (bp) 12467
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Teal-gephyrin
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2973
  • GenBank ID
    268566
  • Tag / Fusion Protein
    • Teal (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsiWI (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)
  • 5′ sequencing primer None
  • 3′ sequencing primer None
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jerry Chen
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid can be used with the low-efficiency Cre in Plasmid 101242: pSin wPGK-Cre ( https://www.addgene.org/101242/ ).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FU- dio Teal-gephyrin - W was a gift from Elly Nedivi (Addgene plasmid # 73918 ; http://n2t.net/addgene:73918 ; RRID:Addgene_73918)
  • For your References section:

    Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo. Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y, So PT, Nedivi E. Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 10.1016/j.neuron.2016.01.010. 10.1016/j.neuron.2016.01.010 PubMed 26853302