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pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2
(Plasmid #74009)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74009 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pminiTol2
  • Backbone size w/o insert (bp) 3459
  • Total vector size (bp) 9668
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    5x UAS
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    382

Gene/Insert 2

  • Gene/Insert name
    Cas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4150

Gene/Insert 3

  • Gene/Insert name
    T2A
  • Insert Size (bp)
    63

Gene/Insert 4

  • Gene/Insert name
    GFP
  • Insert Size (bp)
    714

Gene/Insert 5

  • Gene/Insert name
    U6 promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    331

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 was a gift from Filippo Del Bene (Addgene plasmid # 74009 ; http://n2t.net/addgene:74009 ; RRID:Addgene_74009)
  • For your References section:

    2C-Cas9: a versatile tool for clonal analysis of gene function. Di Donato V, De Santis F, Auer TO, Testa N, Sanchez-Iranzo H, Mercader N, Concordet JP, Del Bene F. Genome Res. 2016 Mar 8. 10.1101/gr.196170.115 PubMed 26957310