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Rlucrarr3-Flash1
(Plasmid #74129)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 74129 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1-RLucC2
  • Backbone manufacturer
    gift from M. Bouvier (University of Montreal)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Arrestin3
  • Alt name
    Arr3, beta-Arrestin2
  • Species
    R. norvegicus (rat)
  • Mutation
    Insertion of FlAsH motif (CCPGCC) following amino acid residue 40.
  • Tag / Fusion Protein
    • Renilla luciferase (rLuc) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site ApaI (unknown if destroyed)
  • 5′ sequencing primer CMV-forward
  • 3′ sequencing primer BGH-Rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pcDNA3.1-rLuc-arrestin3 backbone was a gift from M. Bouvier (University of Montreal, Montreal Quebec, Canada)
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rlucrarr3-Flash1 was a gift from Louis Luttrell (Addgene plasmid # 74129)
  • For your References section:

    The conformational signature of beta-arrestin2 predicts its trafficking and signalling functions. Lee MH, Appleton KM, Strungs EG, Kwon JY, Morinelli TA, Peterson YK, Laporte SA, Luttrell LM. Nature. 2016 Mar 31;531(7596):665-8. doi: 10.1038/nature17154. Epub 2016 Mar 23. 10.1038/nature17154 PubMed 27007854