Purpose(Empty Backbone) To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74223||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||74223-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
Vector backbonepcDNA 3.1
Backbone manufacturerThermo Fisher Scientific
- Backbone size (bp) 6147
Vector typeMammalian Expression
- Promoter CMV
Selectable markersNeomycin (select with G418)
/ Fusion Proteins
- Myc (N terminal on backbone)
- humanized A. aeolicus BioID2 with R40G (N terminal on backbone)
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Information for Cloning Grade DNA (Catalog # 74223-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:myc-BioID2-MCS was a gift from Kyle Roux (Addgene plasmid # 74223 ; http://n2t.net/addgene:74223 ; RRID:Addgene_74223)
For your References section:An improved smaller biotin ligase for BioID proximity labeling. Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844. 10.1091/mbc.E15-12-0844 PubMed 26912792