MCS-BioID2-HA
(Plasmid #74224)

• Purpose: (Empty Backbone) To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
• Depositing Lab: Kyle Roux
• Publication: Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844.

Backbone

• Vector backbone: pcDNA 3.1
• Backbone manufacturer: Thermo Fisher Scientific
• Backbone size (bp): 6113
• Vector type: Mammalian Expression
• Promoter: CMV
• Selectable markers: Neomycin (select with G418)
• Tags / Fusion Proteins:
  • humanized A. aeolicus BioID2 with R40G (C terminal on backbone)
  • HA (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 56 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the location of the BioID2-HA insert in this plasmid is at bp# 951-1673.

Information for Cloning Grade DNA (Catalog # 74224-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here