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MCS-BioID2-HA
(Plasmid #74224)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74224 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA 3.1
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size (bp) 6113
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Selectable markers
    Neomycin (select with G418)
  • Tags / Fusion Proteins
    • humanized A. aeolicus BioID2 with R40G (C terminal on backbone)
    • HA (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

Depositor Comments

Please note that the location of the BioID2-HA insert in this plasmid is at bp# 951-1673.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MCS-BioID2-HA was a gift from Kyle Roux (Addgene plasmid # 74224)
  • For your References section:

    An improved smaller biotin ligase for BioID proximity labeling. Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844. 10.1091/mbc.E15-12-0844 PubMed 26912792