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Purpose(Empty Backbone) To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 74224 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $65 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA 3.1
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Backbone manufacturerThermo Fisher Scientific
- Backbone size (bp) 6113
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Vector typeMammalian Expression
- Promoter CMV
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Selectable markersNeomycin (select with G418)
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Tags
/ Fusion Proteins
- humanized A. aeolicus BioID2 with R40G (C terminal on backbone)
- HA (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Articles Citing this Plasmid
Depositor Comments
Please note that the location of the BioID2-HA insert in this plasmid is at bp# 951-1673.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MCS-BioID2-HA was a gift from Kyle Roux (Addgene plasmid # 74224 ; http://n2t.net/addgene:74224 ; RRID:Addgene_74224) -
For your References section:
An improved smaller biotin ligase for BioID proximity labeling. Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844. 10.1091/mbc.E15-12-0844 PubMed 26912792