MCS-BioID2-HA
(Plasmid #74224)

• Purpose: (Empty Backbone) To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
• Depositing Lab: Kyle Roux
• Publication: Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	74224	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart
Cloning Grade DNA	74224-DNA.cg	2 µg of cloning grade DNA in Tris buffer More Information	1	$95	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pcDNA 3.1
  (Search Vector Database)
• Backbone manufacturer: Thermo Fisher Scientific
• Backbone size (bp): 6113
• Vector type: Mammalian Expression
• Promoter: CMV
• Selectable markers: Neomycin (select with G418)
• Tags / Fusion Proteins:
  • humanized A. aeolicus BioID2 with R40G (C terminal on backbone)
  • HA (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 7 References

Depositor Comments

Please note that the location of the BioID2-HA insert in this plasmid is at bp# 951-1673.

Information for Cloning Grade DNA (Catalog # 74224-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $95 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here