PurposepAAV vector to express PBG (chimeric Glycoprotein) in a Cre-dependent manner
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74287||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Backbone manufacturerK. Deisseroth lab (Stanford)
Growth in Bacteria
Growth Strain(s)NEB Stable
Gene/Insert namePBG (chimeric rabies Glycoprotein)
Speciesglycoprotein for rabies virus SAD B19
- Promoter Ef1a
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer EF1a-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-Ef1a-DIO-PBG-WPRE-hGH was a gift from Edward Callaway (Addgene plasmid # 74287 ; http://n2t.net/addgene:74287 ; RRID:Addgene_74287)
For your References section:Three Types of Cortical Layer 5 Neurons That Differ in Brain-wide Connectivity and Function. Kim EJ, Juavinett AL, Kyubwa EM, Jacobs MW, Callaway EM. Neuron. 2015 Dec 16;88(6):1253-67. doi: 10.1016/j.neuron.2015.11.002. Epub 2015 Dec 6. 10.1016/j.neuron.2015.11.002 PubMed 26671462