pH-tdGFP
(Plasmid #74322)

• Purpose: pH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiae
• Depositing Lab: Joerg Stelling
• Publication: Roberts et al Sci Rep. 2016 Jun 21;6:28166. doi: 10.1038/srep28166.

Backbone

• Vector backbone: pFA6a
• Backbone size w/o insert (bp): 4150
• Total vector size (bp): 5622
• Vector type: Yeast Expression ; yeast tagging
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pH-tdGFP
• Alt name: fluorescent protein derived from sfGFP
• Species: S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 1460
• Mutation: N149Y, Q204H
• Tag / Fusion Protein:
  • none (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry