Purpose(Empty Backbone) an ampR plasmid built from pBluescript: the multiple cloning site (mcs) flanked by inverted head-to-head oriented I-SceI sites. Genbank accession number is KU144822.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74592||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluscript SK+
- Backbone size (bp) 2878
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer GTAAAACGACGGCCAGTGA
- 3′ sequencing primer GGAAACAGCTATGACCATGAT (Common Sequencing Primers)
For sequencing multiple cloning sites (mcs) in the empty vector, linearize the plasmid by cutting at a restriction enzyme site, i.e., EcoRI or BamHI, because strong palindromic sequences flank the mcs make sequencing difficult.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKHR4 was a gift from David Grunwald (Addgene plasmid # 74592 ; http://n2t.net/addgene:74592 ; RRID:Addgene_74592)
For your References section:Precise Editing of the Zebrafish Genome Made Simple and Efficient. Hoshijima K, Jurynec MJ, Grunwald DJ. Dev Cell. 2016 Mar 21;36(6):654-67. doi: 10.1016/j.devcel.2016.02.015. 10.1016/j.devcel.2016.02.015 PubMed 27003937