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AIO-Puro
(Plasmid #74630)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74630 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    All-in-One_Nickase-D10A
  • Backbone size (bp) 9626
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter Cbh
  • Selectable markers
    Puromycin
  • Tag / Fusion Protein
    • Puromycin resistant marker

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Terms and Licenses

Depositor Comments

Puromycin resistance gene is only for transient expression along with Cas9 nickase. Thus, drug selection period should be tested for each experiment. The presence of cellular puromycin resistance could be monitored by performing a control transfection with an empty backbone vector of either AIO-GFP or AIO-mCherry in which GFP/mCherry expression level represents puromycin resistance level.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AIO-Puro was a gift from Steve Jackson (Addgene plasmid # 74630 ; http://n2t.net/addgene:74630 ; RRID:Addgene_74630)
  • For your References section:

    CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing. Chiang TW, le Sage C, Larrieu D, Demir M, Jackson SP. Sci Rep. 2016 Apr 15;6:24356. doi: 10.1038/srep24356. 10.1038/srep24356 PubMed 27079678