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pRubiG-T2A-Cas9
(Plasmid #75348)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 75348 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRubi
  • Backbone size w/o insert (bp) 6939
  • Total vector size (bp) 11985
  • Vector type
    Mammalian Expression, Retroviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Promoter Ubiquitin
  • Tag / Fusion Protein
    • GFP (via t2a) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer Ubi Down
  • 3′ sequencing primer Wre Up
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRubiG-T2A-Cas9 was a gift from Bryan Luikart (Addgene plasmid # 75348 ; http://n2t.net/addgene:75348 ; RRID:Addgene_75348)
  • For your References section:

    A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons. Williams MR, Fricano-Kugler CJ, Getz SA, Skelton PD, Lee J, Rizzuto CP, Geller JS, Li M, Luikart BW. Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611. 10.1038/srep25611 PubMed 27161796