PurposePX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||75349||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 8548
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
gRNA/shRNA sequenceEmpty Cassette
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site BstB1 or Pac1 (not destroyed)
- 3′ cloning site Pac1 (not destroyed)
- 5′ sequencing primer LKO1 (Common Sequencing Primers)
Derived from PX330
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PXL was a gift from Bryan Luikart (Addgene plasmid # 75349 ; http://n2t.net/addgene:75349 ; RRID:Addgene_75349)
For your References section:A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons. Williams MR, Fricano-Kugler CJ, Getz SA, Skelton PD, Lee J, Rizzuto CP, Geller JS, Li M, Luikart BW. Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611. 10.1038/srep25611 PubMed 27161796