WNK3 gRNA (BRDN0001148134)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||76926||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerZhang Lab (Addgene plasmid # 52963)
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
- Promoter hU6
- Cloning method Unknown
- 5′ sequencing primer U6-F
- 3′ sequencing primer pS2-R (Common Sequencing Primers)
The gRNA was designed to target the following Genbank reference sequence(s): NM_001002838.3,NM_020922.4. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:WNK3 gRNA (BRDN0001148134) was a gift from John Doench & David Root (Addgene plasmid # 76926)
For your References section:Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. 10.1038/nbt.3437 PubMed 26780180