Purpose(Empty Backbone) Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78098||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backboneAt U6 promoter driven sgRNA cassette, human codon-optimized SpCas9 gene containing a nuclear localization signal (NLS) and a HA tag at the Cterminus of SpCas9
Vector typePlant Expression, CRISPR
- Promoter U6
Growth in Bacteria
- Cloning method Restriction Enzyme
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
protocol for cloning gRNA can be found in associated article.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHAtC was a gift from Jin-Soo Kim & Sang-Gyu Kim (Addgene plasmid # 78098 ; http://n2t.net/addgene:78098 ; RRID:Addgene_78098)
For your References section:A simple, flexible and high-throughput cloning system for plant genome editing via CRISPR-Cas system. Kim H, Kim ST, Ryu J, Choi MK, Kweon J, Kang BC, Ahn HM, Bae S, Kim JS, Kim SG. J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474. 10.1111/jipb.12474 PubMed 26946469