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pHAtC
(Plasmid #78098)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 78098 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pH2GW7
  • Modifications to backbone
    At U6 promoter driven sgRNA cassette, human codon-optimized SpCas9 gene containing a nuclear localization signal (NLS) and a HA tag at the Cterminus of SpCas9
  • Vector type
    Plant Expression, CRISPR
  • Promoter U6
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Terms and Licenses

Depositor Comments

protocol for cloning gRNA can be found in associated article.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAtC was a gift from Jin-Soo Kim (Addgene plasmid # 78098 ; http://n2t.net/addgene:78098 ; RRID:Addgene_78098)
  • For your References section:

    A simple, flexible and high-throughput cloning system for plant genome editing via CRISPR-Cas system. Kim H, Kim ST, Ryu J, Choi MK, Kweon J, Kang BC, Ahn HM, Bae S, Kim JS, Kim SG. J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474. 10.1111/jipb.12474 PubMed 26946469