pDHJS4 BS-
(Plasmid #78245)

• Purpose: One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).
• Depositing Lab: Tao-shih Hsieh
• Publication: Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60. doi: 10.1093/nar/gks1343. Epub 2012 Dec 28.

Backbone

• Vector backbone: pDHJS3 BS-
• Backbone manufacturer: Addgene #78239
• Backbone size w/o insert (bp): 3360
• Modifications to backbone: inserted attB and attP in place of two loxP sites

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: This plasmid requires a helper phagemid to make single stranded DNA. Suggested helper phaemids: M13K07 (available from NEB) or pM13g (available from Andrew Bradbury at Los Alamos National Laboratory- PMID: 17088290). If using M13K07 helper phagemid, transform plasmid DNA in XL2 MRF'. If using pM13g helper phagemid, transform plasmid DNA in DH5alpha.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: attB
• Insert Size (bp): 48

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: none

Gene/Insert 2

• Gene/Insert name: attP
• Insert Size (bp): 75

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: SphI (not destroyed)
• 3′ cloning site: NdeI (destroyed during cloning)
• 5′ sequencing primer: none

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry