PurposeTo purify GST-tagged recombinant RIPK3 protein
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78827||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
Entrez GeneRIPK3 (a.k.a. RIP3)
- Promoter tac
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX-4T-1-RIPK3 was a gift from Jaewhan Song (Addgene plasmid # 78827 ; http://n2t.net/addgene:78827 ; RRID:Addgene_78827)
For your References section:CHIP controls necroptosis through ubiquitylation- and lysosome-dependent degradation of RIPK3. Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J. Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 2016 Feb 22. 10.1038/ncb3314 PubMed 26900751