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pET30a-AlkB-D135S
(Plasmid #79051)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79051 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET30a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5243
  • Total vector size (bp) 5858
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21 (DE3) for expression
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AlkB
  • Species
    E.coli
  • Insert Size (bp)
    615
  • Mutation
    A truncated AlkB with deletion of the amino (N)-terminal 11 amino acids and D135S mutation
  • GenBank ID
    946708
  • Entrez Gene
    alkB (a.k.a. b2212, ECK2204, aidD)
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30a-AlkB-D135S was a gift from Tao Pan (Addgene plasmid # 79051 ; http://n2t.net/addgene:79051 ; RRID:Addgene_79051)
  • For your References section:

    Efficient and quantitative high-throughput tRNA sequencing. Zheng G, Qin Y, Clark WC, Dai Q, Yi C, He C, Lambowitz AM, Pan T. Nat Methods. 2015 Sep;12(9):835-7. doi: 10.1038/nmeth.3478. Epub 2015 Jul 27. 10.1038/nmeth.3478 PubMed 26214130