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CRY2-INPP5E(D556A)
(Plasmid #79564)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79564 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    mCherry-CRY2PHR
  • Backbone manufacturer
    C. Tucker, University of Colorado Denver
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    INPP5E
  • Species
    H. sapiens (human)
  • Mutation
    C-terminal region, aa 214_644, C641A mutation to delete the C-terminal CAAX box and phosphatase dead D556A mutation
  • Entrez Gene
    INPP5E (a.k.a. CORS1, CPD4, JBTS1, MORMS, PPI5PIV)
  • Promoter CMV
  • Tag / Fusion Protein
    • CRY2 (photolyase homology region domain of Arabidopsis thaliana cryptochrome 2) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

This construct, lacking the N-terminal fluorescent protein, was generated by Nhe1/Not1 digestion to remove mCherry followed by Mungbean nuclease treatment and blunt end ligation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CRY2-INPP5E(D556A) was a gift from Pietro De Camilli & Olof Idevall-Hagren (Addgene plasmid # 79564)
  • For your References section:

    Optogenetic control of phosphoinositide metabolism. Idevall-Hagren O, Dickson EJ, Hille B, Toomre DK, De Camilli P. Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 10.1073/pnas.1211305109. Epub 2012 Jul 30. 10.1073/pnas.1211305109 PubMed 22847441