Purposeexpression of CRY2PHR fused to catalytically dead INPP5E
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79564||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerC. Tucker, University of Colorado Denver
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationC-terminal region, aa 214_644, C641A mutation to delete the C-terminal CAAX box and phosphatase dead D556A mutation
Entrez GeneINPP5E (a.k.a. CORS1, CPD4, JBTS1, MORMS, PPI5PIV, pharbin)
- Promoter CMV
/ Fusion Protein
- CRY2 (photolyase homology region domain of Arabidopsis thaliana cryptochrome 2) (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site PvuI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
This construct, lacking the N-terminal fluorescent protein, was generated by Nhe1/Not1 digestion to remove mCherry followed by Mungbean nuclease treatment and blunt end ligation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CRY2-INPP5E(D556A) was a gift from Pietro De Camilli & Olof Idevall-Hagren (Addgene plasmid # 79564 ; http://n2t.net/addgene:79564 ; RRID:Addgene_79564)
For your References section:Optogenetic control of phosphoinositide metabolism. Idevall-Hagren O, Dickson EJ, Hille B, Toomre DK, De Camilli P. Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 10.1073/pnas.1211305109. Epub 2012 Jul 30. 10.1073/pnas.1211305109 PubMed 22847441