Purposeexpression of mCherry-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79570||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
MutationR445E and R446E; truncation of amino acids 636-661 (please see depositor comments below)
Entrez GenePip5k1c (a.k.a. AI115456, AI835305, Pip5kIgamma)
- Promoter CMV
/ Fusion Proteins
- mCherry (N terminal on backbone)
- CRY2 (photolyase homology region domain of Arabidopsis thaliana cryptochrome 2) (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site PvuI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer mCherry-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Three modifications have been made to PIP5K1g (NM008844): R445E and R446E mutations to favour cytosolic distribution and truncation of amino acids 636-661 to prevent plasma membrane localisation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mCherry-CRY2-P14P5K was a gift from Olof Idevall-Hagren (Addgene plasmid # 79570 ; http://n2t.net/addgene:79570 ; RRID:Addgene_79570)
For your References section:Plasma Membrane Phosphatidylinositol 4,5-Bisphosphate Regulates Ca(2+)-Influx and Insulin Secretion from Pancreatic beta Cells. Xie B, Nguyen PM, Gucek A, Thonig A, Barg S, Idevall-Hagren O. Cell Chem Biol. 2016 Jul 21;23(7):816-26. doi: 10.1016/j.chembiol.2016.06.009. 10.1016/j.chembiol.2016.06.009 PubMed 27447049