pHAGE-E-cadherin-RFP
(Plasmid #79603)

• Purpose: pHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging system
• Depositing Lab: Jeffrey Rosen
• Publication: Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Backbone

• Vector backbone: pHAGE
• Modifications to backbone: Backbone has been significantly modified from original pHAGE vector. Please see full plasmid sequence
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CDH1 promoter region
• Species: H. sapiens (human)
• Insert Size (bp): 1367
• Entrez Gene: CDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pHAGE transfer vectors require Tat to produce virus. Please use a 2nd generation packaging system to make high titer virus with this plasmid.