PurposessAAV genome with Ple254 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79651||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4972
- Total vector size (bp) 7106
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
Gene/Insert namessAAV Ple254-EmGFP WPRE
Alt namePle254 (PAX6 Retinal Enhancer)
SpeciesH. sapiens (human)
Insert Size (bp)2148
- Promoter PAX6 Retinal Enhancer
- Cloning method Restriction Enzyme
- 5′ cloning site See Map (unknown if destroyed)
- 3′ cloning site See Map (unknown if destroyed)
- 5′ sequencing primer M13/pUC Reverse
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEMS2043 was a gift from Elizabeth Simpson (Addgene plasmid # 79651 ; http://n2t.net/addgene:79651 ; RRID:Addgene_79651)
For your References section:PAX6 MiniPromoters drive restricted expression from rAAV in the adult mouse retina. Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, Molday RS, Wasserman WW, Simpson EM. Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 10.1038/mtm.2016.51. eCollection 2016. 10.1038/mtm.2016.51 PubMed 27556059