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pEMS2049
(Plasmid #79657)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79657 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    ssAAV2
  • Backbone size w/o insert (bp) 4972
  • Total vector size (bp) 7105
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ssAAV Ple260-EmGFP WPRE
  • Alt name
    Ple260 (PAX6 retinal enhancer)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2147
  • GenBank ID
  • Promoter PAX6 Retinal Enhancer

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (unknown if destroyed)
  • 3′ cloning site See map (unknown if destroyed)
  • 5′ sequencing primer M13pUC-rev
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS2049 was a gift from Elizabeth Simpson (Addgene plasmid # 79657 ; http://n2t.net/addgene:79657 ; RRID:Addgene_79657)
  • For your References section:

    PAX6 MiniPromoters drive restricted expression from rAAV in the adult mouse retina. Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, Molday RS, Wasserman WW, Simpson EM. Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 10.1038/mtm.2016.51. eCollection 2016. 10.1038/mtm.2016.51 PubMed 27556059