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pssGFP-hCRT
(Plasmid #80022)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80022 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-hCRT
  • Backbone manufacturer
    Eggleton Lab
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRT
  • Alt name
    Calreticulin
  • Species
    H. sapiens (human)
  • Mutation
    signal peptide 17aa added before GFP-CRT, will target to ER
  • Entrez Gene
    CALR (a.k.a. CRT, HEL-S-99n, RO, SSA, cC1qR)
  • Promoter CMV
  • Tags / Fusion Proteins
    • signal peptide (N terminal on backbone)
    • EGFP (N terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pssGFP-hCRT was a gift from Chinten James Lim (Addgene plasmid # 80022 ; http://n2t.net/addgene:80022 ; RRID:Addgene_80022)
  • For your References section:

    alpha-Integrin expression and function modulates presentation of cell surface calreticulin. Liu CC, Leclair P, Monajemi M, Sly LM, Reid GS, Lim CJ. Cell Death Dis. 2016 Jun 16;7:e2268. doi: 10.1038/cddis.2016.176. 10.1038/cddis.2016.176 PubMed 27310876