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(Plasmid #80424)


Item Catalog # Description Quantity Price (USD)
Plasmid 80424 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    May get better pDNA yield if grown at 30 degrees
  • Copy number
    Low Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Mutation
    codon optimized for human
  • Promoter T7
  • Tag / Fusion Protein
    • Geminin (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDNR-SpCas9-Gem was a gift from Sara Howden & Melissa Little (Addgene plasmid # 80424 ; ; RRID:Addgene_80424)
  • For your References section:

    A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells. Howden SE, McColl B, Glaser A, Vadolas J, Petrou S, Little MH, Elefanty AG, Stanley EG. Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4. 10.1016/j.stemcr.2016.07.001 PubMed 27499201