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pTALdNC-AAVS1_T2
(Plasmid #80496)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80496 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDONR221
  • Backbone size w/o insert (bp) 2300
  • Total vector size (bp) 5863
  • Modifications to backbone
    silent mutation to remove Esp3I site in KanR
  • Vector type
    Gateway Entry

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAVS1 TALEN (Right)
  • Species
    Synthetic
  • Insert Size (bp)
    3573
  • Promoter none

Cloning Information

Resource Information

Depositor Comments

Gateway assembled into a custom Gateway Entry vector using Addgene Kit #1000000024

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTALdNC-AAVS1_T2 was a gift from Knut Woltjen (Addgene plasmid # 80496 ; http://n2t.net/addgene:80496 ; RRID:Addgene_80496)
  • For your References section:

    Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives. Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K. Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 10.1016/j.ymeth.2015.12.012. 10.1016/j.ymeth.2015.12.012 PubMed 26707206