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pDGE79
(Plasmid #80581)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80581 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pVM_BGW
  • Backbone manufacturer
    6800
  • Total vector size (bp) 15400
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    pnos:PAT-tnos
  • Alt name
    BASTA resistance, plant selectable
  • Species
    E. coli
  • Insert Size (bp)
    1800

Gene/Insert 2

  • Gene/Insert name
    pPcUbi:Cas9 D10A-tnos
  • Alt name
    Cas9 nickase
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    5300
  • Mutation
    D10A

Gene/Insert 3

  • Gene/Insert name
    BsaI-ccdB_CmR-BsaI
  • Alt name
    dummy
  • Insert Size (bp)
    1500

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDGE79 was a gift from Johannes Stuttmann (Addgene plasmid # 80581 ; http://n2t.net/addgene:80581 ; RRID:Addgene_80581)
  • For your References section:

    Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, Boch J, Stuttmann J. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. 10.1111/tpj.13319 PubMed 27579989