PurposeP2A followed by mKate2 without pA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80828||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepDONR P2R-P3
- Total vector size (bp) 3518
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert namep3E-P2A-mKate2 no pA
- Cloning method Gateway Cloning
- 5′ sequencing primer M13(20)F
- 3′ sequencing primer M13R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p3E-P2A-mKate2 no-pA was a gift from Philip Washbourne (Addgene plasmid # 80828 ; http://n2t.net/addgene:80828 ; RRID:Addgene_80828)
For your References section:A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications. Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne P. PLoS One. 2016 Aug 8;11(8):e0159277. doi: 10.1371/journal.pone.0159277. eCollection 2016. PONE-D-16-19476 [pii] PubMed 27500400