A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications.
Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne P
PLoS One. 2016 Aug 8;11(8):e0159277. doi: 10.1371/journal.pone.0159277. eCollection 2016. PubMed
Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne P
PLoS One. 2016 Aug 8;11(8):e0159277. doi: 10.1371/journal.pone.0159277. eCollection 2016. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 80802 | p5E-CMVmin | minimal cytomegalovirus immediate early enhancer/promoter (CMVmin) from pcDNA3 |
| 80804 | p5E-ESyn1 | hSyn1 promoter fused to CMVmin |
| 80806 | pME-mKate2 no stop | red fluorophore without stop codon |
| 80807 | pME-GFP-P2A | GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide |
| 80808 | pME-nlsGFP-P2A | nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A |
| 80809 | pME-memGFP-P2A | Fyn myristoylation domain fused to N-terminus of GFP (memGFP) without stop codon, followed by P2A |
| 80810 | p3E-mKate2-HA no-pA | hemagglutinin (HA) epitope fused to C-terminus of mKate2 without pA |
| 80812 | p3E-mKate2-myc no-pA | myc epitope fused to C-terminus of mKate2 without pA |
| 80813 | p3E-GFP no-pA | GFP without pA |
| 80814 | p3E-nlsGFP pA | nlsGFP with pA |
| 80815 | p3E-nlsGFP no-pA | nlsGFP without pA |
| 80817 | p3E-GFPmem no-pA | GFPmem without pA |
| 80819 | p3E-CMVmin:GFP no-pA | CMVmin-promoted GFP without pA |
| 80821 | p3E-CMVmin:nlsGFP pA | CMVmin-promoted nlsGFP with pA |
| 80823 | p3E-CMVmin:GFPmem pA | CMVmin-promoted GFPmem with pA |
| 80824 | p3E-CMVmin:GFPmem no-pA | CMVmin-promoted GFPmem without pA |
| 80825 | p3E-P2A-MCS no-pA | P2A followed by multiple cloning site without pA |
| 80826 | p3E-P2A-GFP no-pA | P2A followed by GFP without pA |
| 80827 | p3E-P2A-CFP no-pA | P2A followed by CFP without pA |
| 80828 | p3E-P2A-mKate2 no-pA | P2A followed by mKate2 without pA |
| 82400 | p5E-elavl3 | Gateway-compatible zebrafish elavl3 promoter |
| 82401 | p5E-gfap | Gateway-compatible zebrafish gfap promoter |
| 82402 | p5E-mnx1 | Gateway-compatible zebrafish mnx1 promoter |
| 82404 | pME-tdTomato no stop | Gateway-compatible tdTomato fusion reporter |
| 82405 | pME-BrainbowTEC | Gateway-compatible Brainbow-1.0 with fluorophores tdTomato-myc/EGFP/E2Crimson-HA |
| 82406 | pME-FlEx switch | Gateway-compatible FlEx cassette containing two pairs of heterotypic, antiparallel loxP-type recombination sites |
| 82579 | p5E-hPGK | 5' entry vector containing human phosphoglycerate kinase (hPGK) promoter for ubiquitous expression |
| 82583 | p5E-EF1a/b-actin | 5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression |
| 82586 | p5E-Ui4-eSIBR | 5'entry vector containing human ubiquitin C promoter followed by intronically-expressed eSIBR cassette for ubiquitous expression, RNAi |
| 82589 | p3E-GFP-HA no-pA | 3' entry vector containing HA epitope fused to C-terminus of GFP without pA for imaging, epitope labeling/purification |
| 82590 | p3E-YFP-HA no-pA | 3' entry vector containing HA epitope fused to C-terminus of YFP without pA for imaging, epitope labeling/purification |
| 82591 | p3E-CFP-HA no-pA | 3' entry vector containing HA epitope fused to c-terminus of CFP without pA for imaging, epitope labeling/purification |
| 82592 | p3E-mCherry-HA no-pA | 3' entry vector with HA epitope fused to C-terminus of mCherry without pA for imaging, epitope labeling/purification |
| 82593 | p3E-HA no-pA | 3' entry vector containing HA epitope fused to C-terminus of mCherry without pA for epitope labeling/purification |
| 82594 | p3E-FRB-HA no-pA | 3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerization |
| 82595 | p3E-mCherry-FRB-HA no-pA | 3' entry vector containing HA epitope fused to C-terminus and mCherry fused to N-terminus of FRB without pA for rapamycin/rapalog induced dimerization |
| 82596 | p3E-FRB(KTF)-HA no-pA | 3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (W2101F) without pA for rapamycin/rapalog induced dimerization |
| 82597 | p3E-FRB(PLF)-HA no-pA | 3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerization |
| 82598 | p3E-FKBP-HA no-pA | HA epitope tag fused to C-terminus of human FK506 binding protein 12 (FKBP12) without pA for rapamycin/rapalog induced dimerization |
| 82599 | p3E-SGTAP no-pA | 3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purification |
| 82600 | p3E-Dam-myc no-pA | 3' entry vector containing myc epitope tag fused to C-terminus of E. coli DNA adenine methyltransferase without pA for DamID |
| 82601 | p3E-FRT-KanR-FRT pA | 3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection |
| 84372 | pEpic | 3rd-generation lentiviral destination vector for gateway cloning with puromycin resistance. |
| 84373 | pEpic_Lite | 3rd-generation lentiviral destination vector for gateway cloning |