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Addgene

pME-BrainbowTEC
(Plasmid #82405)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82405 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDONR221

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    tdTomatoMYC fusion
  • Tag / Fusion Protein
    • MYC (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    EGFP

Gene/Insert 3

  • Gene/Insert name
    E2CrimsonHA
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    tdTomato from Roger Tsien, UCSD

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-BrainbowTEC was a gift from Judith Eisen (Addgene plasmid # 82405 ; http://n2t.net/addgene:82405 ; RRID:Addgene_82405)
  • For your References section:

    A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications. Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne P. PLoS One. 2016 Aug 8;11(8):e0159277. doi: 10.1371/journal.pone.0159277. eCollection 2016. PONE-D-16-19476 [pii] PubMed 27500400