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PurposeGateway-compatible Brainbow-1.0 with fluorophores tdTomato-myc/EGFP/E2Crimson-HA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 82405 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nametdTomatoMYC fusion
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Tag
/ Fusion Protein
- MYC (C terminal on insert)
Gene/Insert 2
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Gene/Insert nameEGFP
Gene/Insert 3
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Gene/Insert nameE2CrimsonHA
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made bytdTomato from Roger Tsien, UCSD
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-BrainbowTEC was a gift from Judith Eisen (Addgene plasmid # 82405 ; http://n2t.net/addgene:82405 ; RRID:Addgene_82405) -
For your References section:
A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications. Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne P. PLoS One. 2016 Aug 8;11(8):e0159277. doi: 10.1371/journal.pone.0159277. eCollection 2016. PONE-D-16-19476 [pii] PubMed 27500400