Purposeexpresses a constitutively active mutant of 4E-BP1 under the CAG promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||81122||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4802
- Total vector size (bp) 5150
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name4EBP1 F113A
Alt nameEif4ebp1 mutant (F113A)
SpeciesM. musculus (mouse)
Entrez GeneEif4ebp1 (a.k.a. 4e-bp1, AA959816, PHAS-I)
- Promoter CAG
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were also used in the following publication "Normalizing translation through 4E-BP prevents mTOR-driven cortical mislamination and ameliorates aberrant neuron integration. Lin TV, Hsieh L, Kimura T, Malone TJ, Bordey A. Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):11330-11335."
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAGGS-4EBP1 F113A was a gift from Angelique Bordey (Addgene plasmid # 81122 ; http://n2t.net/addgene:81122 ; RRID:Addgene_81122)
For your References section:mTORC1 targets the translational repressor 4E-BP2, but not S6 kinase 1/2, to regulate neural stem cell self-renewal in vivo. Hartman NW, Lin TV, Zhang L, Paquelet GE, Feliciano DM, Bordey A. Cell Rep. 2013 Oct 31;5(2):433-44. doi: 10.1016/j.celrep.2013.09.017. Epub 2013 Oct 17. 10.1016/j.celrep.2013.09.017 PubMed 24139800