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pICE-HA-MRE11-WT
(Plasmid #82033)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 82033 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pICE
  • Backbone manufacturer
    Synthetic - Addgene Plasmid #46960
  • Backbone size w/o insert (bp) 5023
  • Total vector size (bp) 7150
  • Modifications to backbone
    HA Tag and cloning sites
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MRE11
  • Alt name
    MRE11A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2127
  • Promoter CMV-tet
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pICE-HA-MRE11-WT was a gift from Patrick Calsou (Addgene plasmid # 82033 ; http://n2t.net/addgene:82033 ; RRID:Addgene_82033)
  • For your References section:

    Coordinated nuclease activities counteract Ku at single-ended DNA double-strand breaks. Chanut P, Britton S, Coates J, Jackson SP, Calsou P. Nat Commun. 2016 Sep 19;7:12889. doi: 10.1038/ncomms12889. 10.1038/ncomms12889 PubMed 27641979