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FLAG-ZnT1-WT
(Plasmid #82340)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 82340 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV-FLAG10
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 6500
  • Total vector size (bp) 8000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZnT1-WT
  • Alt name
    SLC30A1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Entrez Gene
    SLC30A1 (a.k.a. ZNT1, ZRC1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site Kpn1 (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer ZnT1-internal
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Entry clone for ZnT1-wild type obtained from DNASU. ZnT1 insert cloned into pCMV-FLAG10 using Not1 and Kpn1 restriction enzymes
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-ZnT1-WT was a gift from Somshuvra Mukhopadhyay (Addgene plasmid # 82340 ; http://n2t.net/addgene:82340 ; RRID:Addgene_82340)
  • For your References section:

    Structural Elements in the Transmembrane and Cytoplasmic Domains of the Metal Transporter SLC30A10 Are Required for Its Manganese Efflux Activity. Zogzas CE, Aschner M, Mukhopadhyay S. J Biol Chem. 2016 Jul 29;291(31):15940-57. doi: 10.1074/jbc.M116.726935. Epub 2016 Jun 15. 10.1074/jbc.M116.726935 PubMed 27307044