Holiday Schedule: Addgene will be closed December 22nd & 25th and January 1st. For details, see our holiday shipping schedule. If you have any questions, please contact us at [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

ECON
(Plasmid #82553)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 82553 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHTN HaloTag(R) CMV-neo
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    DNMT3A
  • Species
    H. sapiens (human)
  • Entrez Gene
    DNMT3A (a.k.a. DNMT3A2, M.HsaIIIA, TBRS)
  • Promoter CMV
  • Tag / Fusion Protein
    • HALO (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    CRY2
  • Species
    A. thaliana (mustard weed)
  • Mutation
    N-terminal photolyase homology region domain of CRY2 only
  • Entrez Gene
    CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ECON was a gift from Joseph Irudayaraj (Addgene plasmid # 82553)
  • For your References section:

    Optogenetic regulation of site-specific subtelomeric DNAmethylation. Choudhury SR, Cui Y, Narayanan A, Gilley DP, Huda N, Lo CL, Zhou FC, Yernool D, Irudayaraj J. Oncotarget. 2016 Jul 4. doi: 10.18632/oncotarget.10394. 10.18632/oncotarget.10394 PubMed 27391261