Purpose5' entry vector containing the zebrafish krt5 promoter for epidermis-specific expression
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||82585||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepDONR P4-P1R
- Total vector size (bp) 5068
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namezebrafish krt5 promoter
SpeciesD. rerio (zebrafish)
- Promoter None
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
This plasmid was also used in Fowler et al., 2016 (PMID: 27500400) and is part of the associated kit.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p5E-krt5 was a gift from Kryn Stankunas (Addgene plasmid # 82585 ; http://n2t.net/addgene:82585 ; RRID:Addgene_82585)
For your References section:Spatial and temporal control of transgene expression in zebrafish. Akerberg AA, Stewart S, Stankunas K. PLoS One. 2014 Mar 18;9(3):e92217. doi: 10.1371/journal.pone.0092217. eCollection 2014. PONE-D-13-46380 [pii] PubMed 24643048