Addgene - pcdna3.1 hciap1mut Plasmid Data

Plasmid 8337: pcdna3.1 hciap1mut

Gene/insert name:
ciap1mut
Alt name:
cIAP1
Insert size:
1700
Species:
H. sapiens (human)
Entrez Gene:
BIRC2 (API1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1)
Fusion protein or tag:
myc
Terminal:
N terminal on backbone
Mutation:
H588A
Vector backbone:
pcDNA3.1
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Jon Ashwell
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: TNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2. Li et al (Nature 2002 Mar 21;416(6878):345-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8337" in your Materials and Methods section.