|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8398||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4070
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1785
Entrez GeneMyod1 (a.k.a. AI503393, MYF3, Myo, MyoD, Myod-, Myod-1, bHLHc, bHLHc1)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
CMV promoter/SV40 splice and polyA sites. See author's map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CMV-MyoD was a gift from Andrew Lassar (Addgene plasmid # 8398 ; http://n2t.net/addgene:8398 ; RRID:Addgene_8398)
For your References section:Cyclin-mediated inhibition of muscle gene expression via a mechanism that is independent of pRB hyperphosphorylation. Skapek SX, Rhee J, Kim PS, Novitch BG, Lassar AB. Mol Cell Biol 1996 Dec;16(12):7043-53. PubMed 8943359