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pCAG-Neo-PuroDTK
(Plasmid #84036)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84036 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    5'-PTK-3'
  • Backbone manufacturer
    Wellcome trust Sanger Institute
  • Modifications to backbone
    Insert CAG promoter, Neomycin gene
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Promoter CAG promoter
  • Selectable markers
    Neomycin (select with G418), Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer T7:taatacgactcactatagg
  • 3′ sequencing primer T3: aattaaccctcactaaagg
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The vector backbone is gifted from Dr. Allan Bradley
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-Neo-PuroDTK was a gift from Yuet Wai Kan (Addgene plasmid # 84036 ; http://n2t.net/addgene:84036 ; RRID:Addgene_84036)
  • For your References section:

    Seamless modification of wild-type induced pluripotent stem cells to the natural CCR5Delta32 mutation confers resistance to HIV infection. Ye L, Wang J, Beyer AI, Teque F, Cradick TJ, Qi Z, Chang JC, Bao G, Muench MO, Yu J, Levy JA, Kan YW. Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9591-6. doi: 10.1073/pnas.1407473111. Epub 2014 Jun 9. 10.1073/pnas.1407473111 PubMed 24927590